Book description
Designed as a research-level guide to current strategies and methods of
membrane protein production on the small to intermediate scale, this
practice-oriented book provides detailed, step-by-step laboratory
protocols as well as an explanation of the principles behind each
method, together with a discussion of its relative advantages and
disadvantages.
Following an introductory section on current challenges in membrane
protein production, the book goes on to look at expression systems,
emerging methods and approaches, and protein specific considerations.
Case studies illustrate how to select or sample the optimal production
system for any desired membrane protein, saving both time and money on
the laboratory as well as the technical production scale.
Unique in its coverage of "difficult" proteins with large
membrane-embedded domains, proteins from extremophiles, peripheral
membrane proteins, and protein fragments.
Anne Skaja Robinson is Professor and Associate Chair for Biochemical
Engineering in the Department of Chemical Engineering at the University
of Delaware in Newark, Delaware, USA. She obtained her Bachelor's and
Master's degrees from The Johns Hopkins University, and her Ph. D. from
the University of Illinois at Urbana-Champaign. Prior to joining the
faculty at Delaware in 1997, she was a postdoctoral fellow in the
Department of Biology at MIT. She is a widely acknowledged leader in the
field of protein production, and has authored over 50 scientific
publications. Also, she is the recipient of numerous awards, including
NSF's PECASE/Career award, the DuPont Young Professor award, and is an
2011 AIMBE Fellow. She is a member of the Editorial Boards of
Biotechnology and Bioengineering, and Biotechnology Journal, and the
former Chair of the American Chemical Society BIOT division.
