Book description
Discover how to use HILIC to analyze and better understand polar compounds
An increasingly popular analytical method, hydrophilic interaction
chromatography (HILIC) has the ability to retain and separate polar
compounds that are often difficult to analyze by reversed-phase
high-performance liquid chromatography (HPLC) or other analytical
methods. Offering a comprehensive review, this book enables readers to
develop a fundamental understanding of how HILIC works and then apply
that knowledge to develop and implement a variety of practical applications.
Hydrophilic Interaction Chromatography begins with discussions
of HILIC retention mechanisms, stationary phases, and general method
development. This sets the foundation for the book's extensive
coverage of applications. The authors address unique separation
challenges for bioanalytical, environmental, pharmaceutical, and
biochemical applications. Moreover, there is a thorough discussion of
HILIC in two-dimensional chromatography.
With contributions from leading analytical scientists who have
extensive experience in HILIC as well as HPLC, Hydrophilic
Interaction Chromatography serves as a practical guide for
researchers, featuring:
- Detailed examples of HILIC methods and development approaches
- Thorough explanations of retention mechanisms and the impact of
stationary phase and mobile phase properties on separations
- Step-by-step guidance for developing efficient, sensitive, and
robust HILIC methods
- References to the primary literature at the end of each chapter
Hydrophilic Interaction Chromatography is written for
scientists who use or develop analytical methods for the separation of
polar compounds. In particular, these researchers will discover how
HILIC can be used to analyze and better understand the composition of
pharmaceutical, bioanalytical, biochemical, chemical, food, and
environmental samples.
BERNARD A. OLSEN, PhD, has close to three decades of experience
at Eli Lilly and Company in chemistry, manufacturing, and control of
drug substances and drug products. He contributed to the development
and support of more than twenty-five commercialized drugs as well as
numerous developmental drugs. A Fellow of the American Association of
Pharmaceutical Scientists, Dr. Olsen currently provides consulting and
training services to the pharmaceutical industry and serves as Chair
of the USP Expert Committee on Monograph Development: Small Molecules 3.
BRIAN W. PACK, PhD, is a Research Advisor in Analytical
Sciences R&D at Eli Lilly and Company, where he has contributed to
the development of many solid oral and parenteral dosage forms. He is
recognized for his contributions to HPLC method development, raw
material control strategies, genotoxic impurities, cleaning
validation, and colorimetry. Dr. Pack has published on the topics of
HILIC applications, Raman spectroscopy, mass spectrometry,
dissolution, and cleaning verification.
