Book description
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition
provides a coherent introduction to the principles and applications of
the integrated optical microscope system, covering both theoretical and
practical considerations. It expands and updates discussions of
multi-spectral imaging, intensified digital cameras, signal
colocalization, and uses of objectives, and offers guidance in the
selection of microscopes and electronic cameras, as well as appropriate
auxiliary optical systems and fluorescent tags.
The book is divided into three sections covering optical principles in
diffraction and image formation, basic modes of light microscopy, and
components of modern electronic imaging systems and image processing
operations. Each chapter introduces relevant theory, followed by
descriptions of instrument alignment and image interpretation. This
revision includes new chapters on live cell imaging, measurement of
protein dynamics, deconvolution microscopy, and interference microscopy.
DOUGLAS B. MURPHY supervises core facilities in microscopy and
histology at the new HHMI Janelia Farm Research Campus in Ashburn,
Virginia. An Adjunct Professor of Cell Biology at Johns Hopkins School
of Medicine in Baltimore, Maryland, Dr. Murphy helped establish the
School of Medicine Microscope Facility there, which he supervised
until 2006.
MICHAEL W. DAVIDSON is an assistant scholar/scientist
affiliated with the National High Magnetic Field Laboratory and the
Department of Biological Science at Florida State University where he
is involved in developing educational websites. His digital images and
photomicrographs have graced the covers of over 2,000 publications.
