Book description
Phosphorylation is the addition of a phosphate (PO
4
) group to a protein or other organic molecule. Phosphorylation
activates or deactivates many protein enzymes, causing or preventing the
mechanisms of diseases such as cancer and diabetes. This book shows how
to use mass spectrometry to determine whether or not a protein has been
correctly modified by the addition of a phosphate group. It also
provides a combination of detailed, step-by-step methodology for
phosphoproteomic sample preparation, mass spectral instrumental
analysis, and data interpretation approaches. Furthermore, it includes
the use of bioinformatic Internet tools such as the Blast2GO gene
ontology (GO) tool, used to help understand and interpret complex data
collected in these studies.
BRYAN M. HAM, PhD, is a member of the
American Society for Mass Spectrometry and the American Chemical
Society. He has conducted proteomics and lipidomics research at The
Ohio State University and Pacific Northwest National Laboratory in
Richland, Washington. He is currently working for the Department of
Homeland Security at the U. S. Customs and Border Protection New York
Laboratory. He has published numerous research papers in peer-reviewed
journals, and is the author of Even Electron Mass Spectrometry with
Biomolecule Applications (Wiley).
